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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were documented but comprehensive nucleotide sequence information and facts is just not however accessible.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.
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This guidebook outlines additional attainable optimization of mobile and immunoassay parameters to make certain the very best results are...
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This information outlines further more achievable optimization of cellular and immunoassay parameters to guarantee the very best success are...
Antibodies sure to the blocking peptide now not bind to the epitope on the goal protein. By evaluating the staining within the blocked antibody as opposed to the antibody alone, one can see which staining is particular.
Focus on Facts Cyclin E belongs towards the hugely conserved click here cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinct expression and degradation styles which add into the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates for the G1-S section boundary and is particularly degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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